High frequency somatic embryogenesis and plant regeneration in petiole and leaf explant cultures and petiole-derived embryogenic cell suspension cultures of Hylomecon vernalis |
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Authors: | Kim SW In DS Kim TJ Liu JR |
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Institution: | (1) KCTC, Genetic Resources Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), P.O. Box 115, Taejon, 305-333, South Korea;(2) Eugentech Inc, BVC 112, 52 Oun-dong, Yusong, Taejon, 305-333, South Korea;(3) Plant Cell Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology (KRIBB), P.O. Box 115, Taejon, 305-333, South Korea |
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Abstract: | Culture conditions are described for high frequency somatic embryogenesis and plant regeneration in petiole and leaf explant cultures and petiole-derived embryogenic cell suspension cultures of Hylomecon vernalis Max. Petiole explants formed embryogenic calluses at a frequency of 53% when cultured on B5 medium supplemented with 13.6 M 2,4-dichlorophenoxyacetic acid (2,4-D) alone. Leaf explants formed embryogenic calluses at a frequency of 21% when cultured at a combination of 4.52 M 2,4-D and 2.22 M 6-benzyladenine. Cell suspension cultures were established with petiole-derived embryogenic calluses using liquid B5 medium with 4.52 M 2,4-D. Upon plating onto B5 basal medium, cell suspension cultures produced numerous somatic embryos, which then developed into plantlets. Regenerated plantlets were transplanted to potting soil and grown to maturity in a greenhouse. |
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Keywords: | medicinal plant Papaveracea somatic embryo |
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