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Exploring the multi-drug resistance in Escherichia coli O157:H7 by gene interaction network: A systems biology approach |
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| Authors: | Sravan Kumar Miryala Sudha Ramaiah |
| Institution: | Medical and Biological Computing Laboratory, School of Biosciences and Technology, Vellore Institute of Technology (VIT), Vellore 632014, Tamil Nadu, India. |
| Abstract: | In the present study, we have constructed an interaction network of 29 antibiotic resistant genes along with 777 interactions in E. coli O157:H7. Gene ontology analysis reveals that 94, 89 and 67 genes have roles in the cellular process, biological process and molecular function respectively. Gene complexes related to tripartite efflux pumps mdtEF-tolC and ABC family efflux pump macAB-tolC play key roles in multidrug efflux systems. It is noteworthy to mention that, 19 genes are involved in multi-efflux pumps and they play a significant role in multidrug resistance (MDR); while 18 genes are vital for fatty acid synthesis. Interestingly, we found that the four genes arnABCD are involved in both MDR and in fatty acid synthesis. Hence these genes could be targeted for new drug discovery. On the whole, our results provide a detailed understanding of the mode of MDR mechanisms in E.coli O157:H7. |
| Keywords: | Corresponding author at: Department of Bio-Sciences Vellore Institute of Technology (VIT) Vellore India Gene network Multidrug resistance Antimicrobial resistance gene Clustering analysis AMR Antimicrobial resistance ARDB Antibiotic resistant genes Database BP Biological processes CC Cellular components COG Clusters of Orthologous Groups EP Efflux pump GM GeneMania GO Gene Ontology HUS Hemolytic Uremic Syndrome KEGG Kyoto Encyclopaedia of Genes and Genomics MCODE Molecular Complex Detection MDR Multidrug Resistance MF Molecular Functions OM Outer membrane RND-pump Resistance-nodulation-division pump STRING Search Tool for the Retrieval of Interacting Genes/Proteins |
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