Cloning and promoter analysis of the cotton lipid transfer protein gene Ltp3(1) |
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Authors: | Liu H C Creech R G Jenkins J N Ma D P |
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Institution: | Department of Biochemistry and Molecular Biology, Box 9650, Mississippi State University, Mississippi State, MS 39762, USA. |
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Abstract: | A cotton Ltp3 gene and its 5' and 3' flanking regions have been cloned with a PCR-based genomic DNA walking method. The amplified 2.6 kb DNA fragment contains sequences corresponding to GH3 cDNA which has been shown to encode a lipid transfer protein (LTP3). The gene has an intron of 80 bp which is located in the region corresponding to the C-terminus of LTP3. The Ltp3 promoter was systematically analyzed in transgenic tobacco plants by employing the Escherichia coli beta-glucuronidase gene (GUS) as a reporter. The results of histochemical and fluorogenic GUS assays indicate that the 5' flanking region of the Ltp3 gene contains cis-elements conferring the trichome specific activity of Ltp3 promoter. |
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