Fatty acid efficiency profile in uncoupling of Acanthamoeba castellanii mitochondria |
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| Authors: | Aleksandra Swida Małgorzata Czarna Andrzej Woyda-Płoszczyca Anna Kicinska Francis E. Sluse Wieslawa Jarmuszkiewicz |
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| Affiliation: | (1) Laboratory of Bioenergetics, Adam Mickiewicz University, Fredry 10, 61-701, Poznan, Poland;(2) Laboratory of Bioenergetics and Molecular Physiology, Liege University, Sart Tilman, B-4000, Liege, Belgium |
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| Abstract: | A profile of free fatty acid (FFA) specificity in Acanthamoeba castellanii mitochondrial uncoupling is described. The FFA uncoupling specificity was observed as different abilities to stimulate resting respiration, to decrease resting membrane potential, and to decrease oxidative phosphorylation efficiency. Tested unsaturated FFA (C18–20) were more effective as uncouplers and protonophores when compared to tested saturated FFA (C8–18), with palmitic acid (C16:0) as the most active. As FFA efficiency in mitochondrial uncoupling is related to physiological changes of fatty acid composition (and thereby FFA availability) during growth of amoeba cells, it could be a way to regulate the activity of an uncoupling protein and thereby the efficiency of oxidative phosphorylation during a cell life of this unicellular organism. Aleksandra Swida and Małgorzata Czarna contributed equally to this work. |
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| Keywords: | Fatty acids Uncoupling Uncoupling protein Mitochondria Acanthamoeba castellanii |
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