荧光法研究血清白蛋白与药物的结合作用

本文应用荧光光谱法,观测了药物分子头孢菌素Ⅳ、异烟肼、维生素B_6和氟哌酸对白蛋白荧光的猝灭。由Lineweaver-Burk双倒数作图法,确定了药物与白蛋白作用的离解常数。并通过Forster偶极-偶极无辐射能量转移机理确定了药物分子氟哌酸在人血清白蛋白中与色氨酸残基之间的距离R为2.55nm,由这一距离确定了药物分子可能进入的区域和位置。

Study on the Interaction of Serum Albumin and Drugs by the Fluorescence Method

In this papers, applying fluorescence method, we observed the fluorescence quenching of human serum albumin by its interaction with the drugs cephalo-sporin IV, isoniazide,vitamin B6 and norfloxacin.The interaction dissociation constants of human serum albumin and drug have been determined from a double reciprocal Lineweaver-Burk plot.From the Forster dipole-dipole energy transfer, the distance between the drug norfloxacin and the 214th tryptophane to be measured is 2.55nm.From this distance, we can determine the region and binding site of drug in human serum albumin.