Glutathione-supplemented tris-citric acid extender improves the post-thaw quality and in vivo fertility of buffalo (Bubalus bubalis) bull spermatozoa |
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| Authors: | Muhammad S Ansari Bushra A Rakha Syed MH Andrabi Nemat Ullah Razia Iqbal William V Holt Shamim Akhter |
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| Institution: | 1. Department of Zoology, University of Gujrat, Gujrat 50700, Pakistan;2. Animal Physiology Laboratory, Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Pakistan;3. Institute of Zoology, Zoological Society of London, Regent''s Park, London, UK;1. ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana, India;2. Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India;1. Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana 125001, India;2. Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India;1. ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana, India;2. Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India;1. Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran;2. Department of Animal Science, Faculty of Agriculture Science, Lorestan University, Khorramabad, Iran;3. Research Center for New Technology in Life Science Engineering, University of Tehran, Iran;4. Department of Animal and Poultry Science, College of Aburaihan, University of Tehran, Pakdasht, Tehran, Iran;5. Department of Poultry Sciences, Faculty of Agriculture, Tarbiat Modarres University, Tehran, Iran |
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| Abstract: | In this study we evaluated the effects of semen extender supplementation with different concentrations of glutathione (GSH) on buffalo (Bubalus bubalis) bull sperm motility, plasma membrane integrity, viability and DNA integrity as well as in vivo fertility. Semen from three Nili-Ravi buffalo bulls was collected, and qualified semen ejaculates (n = 18) were split into five aliquots for dilution (37 °C; 50 × 106 spermatozoa ml?1) with experimental tris-citric acid extender containing 0, 0.5, 1.0, 1.5 or 2.0 mM GSH. Extended semen was cooled to 4 °C, equilibrated and filled in French straws. The straws were kept on liquid nitrogen vapors (5 cm above the LN2 level) for 10 min and plunged in liquid nitrogen for storage. Sperm motility (%), plasma membrane integrity (%), viability (%) and DNA integrity (%) were assessed at 0, 2 and 4 h post-thawing (37 °C). Extender supplementation with GSH (0.5, 1.0, 1.5 and 2.0 mM) increased sperm motility, plasma membrane integrity and viability in a dose dependent manner. Sperm DNA integrity was higher (p < 0.05) in all experimental extenders containing GSH when compared to the control extender (0 mM GSH). The in vivo fertility rate of cryopreserved buffalo bull (n = 2) spermatozoa was higher (p < 0.05) in extender containing 2.0 mM GSH compared to that of control. In summary, tris-citric acid extender supplemented with glutathione improved the freezability of buffalo bull spermatozoa in a dose dependant manner. Moreover, the addition of 2.0 mM GSH to the extender enhanced the in vivo fertility of buffalo (Bubalus bubalis) bull spermatozoa. |
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