蒙脱石对细菌黏附Caco-2细胞的影响

采用Caco-2细胞培养模型,观察两歧双歧杆菌、嗜酸乳杆菌、嗜水气单胞菌、副溶血弧菌、大肠杆菌、鼠伤寒沙门菌的黏附率,并在培养液中加入蒙脱石,计算蒙脱石对细菌黏附的阻断率,探讨蒙脱石对上述细菌黏附作用的影响。结果表明:所试菌与Caco-2细胞均有不同程度的黏附作用;蒙脱石对细菌黏附Caco-2细胞均有不同程度的阻断作用,对病原菌黏附Caco-2细胞的阻断作用要明显大于其对益生菌的阻断效果,其中对大肠杆菌、鼠伤寒沙门菌、嗜水气单胞菌、副溶血弧菌黏附的阻断率分别为54.22%、48.41%、60.53%、50.64%,而对两歧双歧杆菌、嗜酸乳杆菌黏附的阻断率分别为25.64%和21.49%。结果提示蒙脱石可有效阻断病原菌黏附,从而防治肠道细菌感染和细菌移位。

Effects of Montmorillonite on Bacterial Adhesion to Caco-2 Cells

In order to investigate the adhesion of probiotic or pathogenic strains to intestina epithelial cells and the effects of montmorillonite (MMT) on the bacterial adhesion, Caco-2 cell line was used as an in vitro model for intestinal epithelium and bacterial adhesion to Caco-2 cell cultures was quantitated using radiolabelled bacteria. The results showed that the adhesion percentage of Bifidobacterium bifidum to Caco-2 cell was significantly higher than Escherichia coli, Salmonella typhimurium, Aeromonas hydrophila and Vibrio parahaemolyticu. The ability of MMT to inhibit adhesion of pathogenic strains to Caco-2 cells was higher than that of probiotic strains. The percent- ages of MMT against adhesion of Escherichia coli, Salmonella typhimurium, Aeromonas hydrophila, Vibrio parahaemolyticus to Caco-2 cell were 54.22%, 48.41%, 60.53% and 50.64%, respectively, while against Bifidobacterium bifidum and Lactobacillus acidophilus were 25.64% and 21.49%, respectively.