|
|||||
|
|
| 气道平滑肌细胞Ryanodine受体亚型mRNA的表达及其在哮喘中的变化 | |
| 作者姓名： | Wang Y Zhang SY Wang XL Qian GS |
| 作者单位： | 1. 第三军医大学新桥医院呼吸研究所,重庆,400037 2. 第二炮兵总医院呼吸科,北京,100088 3. 军事医学科学院微生物流行病研究所免疫室,北京,100071 |
| 摘 要： | 目的:测定正常大鼠气道平滑肌细胞(ASMCs)中Ryanodine受体(Ryanodine receptor RyR)各亚型mRNA的表达以及在哮喘中的变化.方法:采用胶原酶消化法培养大鼠ASMCs,RT-PCR测定受体各亚型mRNA的表达.卵蛋白致敏并激发大鼠制备慢性哮喘模型,RT-PCR测定各受体mRNA表达水平的变化.结果:大鼠ASMCs中可见RyR1、RyR2和RyR3三种RyR亚型mRNA共表达,扩增片段测序结果与GenBank中登录的大鼠RyR1-3序列完全一致.制备的慢性哮喘模型中,病理切片显示平滑肌层及黏膜层明显增厚,RyR1的表达较对照组有明显的上调P＜0.05.结论:大鼠气道平滑肌细胞中RyR三种亚型共表达,提示存在着复杂的细胞内钙调节机制;并且RyR1在慢性哮喘的发生过程中可能起了一定的作用. |
| 关 键 词： | 受体 气道平滑肌细胞 逆转录-聚合酶链式反应 大鼠 |
| 文章编号： | 1000-6834(2004)02-0181-04 |
| 修稿时间： | 2003年7月14日 |
Expression of ryanodine receptor isoforms in airway smooth muscle cells of rats |
|
| Wang Y,Zhang SY,Wang XL,Qian GS.Expression of ryanodine receptor isoforms in airway smooth muscle cells of rats[J].Chinese Journal of Applied Physiology,2004,20(2):181-184. | |
| Authors: | Wang Ying Zhang Sui-yang Wang Xi-liang Qian Gui-sheng |
| Institution: | WANG Ying1,ZHANG Sui-yang2,WANG Xi-liang3,QIAN Gui-sheng1 |
| Abstract: | Aim: To detect the expression of ryanodine receptor(RyR) subtypes in normol rat airway smooth muscle cells(ASMCs) and changes during chronic asthma formation. Methods: ASMCs were cultured with collagen enzyme digestion method. The expression of subtyes of RyR were detected by RT-PCR. Purified PCR product linked with pGEM-T vector to make DNA sequence assay. Chronic asthma model was made with OVA, the changes of RyRs detected by RT-PCR. Results: All subtypes of RyR were expressed in airway smooth muscle cells of normol rat. The expression of RyR1 increased obviously compared with control group(P<0.05) on chronic asthma.Conclusion: Co-expression of three subtypes of RyR in ASMCs of normal rat, indicate that there are complicated intercellular Ca 2 regulation mechanism in ASM, moreover RyR1 might play a role during asthma development. |
| Keywords: | Ryanodine |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |