天麻大型细胞消化蜜环菌过程中溶酶体小泡的作用

蜜环菌(Armillaria mellea Fr.)菌丝由天麻(Gastrodia elata Bl.)皮层细胞经纹孔侵入大型细胞。初期大型细胞的原生质膜凹陷,同时细胞壁产生乳突状加厚阻止菌丝侵入。当菌丝侵入大型细胞以后,凹陷的质膜将菌丝紧密包围,大量由单位膜围成的小泡聚集在其周围。随后这些小泡的膜与质膜融合并将其内含物释放到菌丝周围的空间中,凹陷质膜逐渐膨大成为一个包围菌丝的消化泡。小泡和消化泡中均具酸性磷酸酶活性反应产物,证实其分别相当于植物溶酶体系统中的初级和次级溶酶体。菌丝在消化泡中被彻底消化。

ROLE OF LYSOSOMAL VESICLES IN THE DIGESTIVE PROCESS OF ARMILLARIA MELLEA BY THE LARGE CELLS OF GASTRODIA ELATA

The hyphae of Armillaria mellea Fr.invade the large cells of Gastrodia data B1.through the wall pits of cortical cells.During early stage the plasmalemma of large cell invaginates and the cell wall forms papillary thickenings to restrain the hyphae from invading.When a hypha enters a large cell,it is encircled tightly by the invaginated plasmalemma which is surrounded by a large number of vesicles coated by a unit membrane.As these vesicles fusing with their me- mbranes to the plasmalemma and discharging their contents into the space around the hypha,the space lined by the invaginated plasmalemma enlarges gradually and becomes a digestive va- cuole in which a hypha is completely digested.Reaction product form acid phosphatase activi- ties in the vesicles and digestive vacuoles testifies that the vesicles and digestive vacuoles are identical with primary and secondary lysosomes of plant lysosomal system respectively.