| 中美有机磷杀虫药剂抗性库蚊复合种群酯酶基因扩增的研究(英文) |
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| 引用本文： | 乔传令.中美有机磷杀虫药剂抗性库蚊复合种群酯酶基因扩增的研究(英文)[J].Entomologia Sinica,1996(2). |
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| 作者姓名： | 乔传令 |
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| 作者单位： | 中国科学院动物研究所 北京100080 |
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| 基金项目： | This project was supported in part by EEC subsidies and by National Natural Science Fundation of China. |
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| 摘 要： | 用脉冲电场凝胶电泳技术,从北京和美国加州的有机磷杀虫药剂抗性库蚊复合品系中分离出一条49kb的酯酶基因扩增片段。该片段可能是一个完整的扩增单元,也是迄今分离出的最大的一条昆虫酯酶基因扩增片段,扩增基因结构的研究正在进行之中。本文还重点讨论了DNA大片段的制备方法和脉冲电场电泳分离技术。
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STUDIES OF ESTERASE GENE AMPLIFICATION IN ORGANOPHOSPHATE RESISTANT CULEX PIPIENS COMPLEX FROM AMERICA AND CHINA |
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| Authors: | Chuanling QiaoInstitute of Zoology Academia Sinica Beijing China |
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| Institution: | Chuanling QiaoInstitute of Zoology,Academia Sinica,Beijing,100080 China |
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| Abstract: | Pulsed-field gel electrophoresis analysis of Beijing and Californian populations of Culex pipiens complex mosquitoes revealed that the enzyme Mlul produced a band of 49kb. This probably is one whole amplification unit, the largest fragment found so far in the mosquitoes. The method for the preparation of large DNA molecules was described and the electrophoretic condition for the separation of large DNA molecules is discussed. |
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| Keywords: | Culex pipiens mosquitoes pulsed-field gel electrophoresis insecticide resistance |
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