Utilization of a zebra finch BAC library to determine the structure of an avian androgen receptor genomic region |
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Authors: | Luo Meizhong Yu Yeisoo Kim Hyeran Kudrna Dave Itoh Yuichiro Agate Robert J Melamed Esther Goicoechea José L Talag Jayson Mueller Christopher Wang Wenming Currie Jennifer Sisneros Nicholas B Wing Rod A Arnold Arthur P |
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Institution: | Department of Plant Sciences, Arizona Genomics Institute, University of Arizona, Tucson, AZ 85721, USA. |
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Abstract: | The zebra finch (Taeniopygia guttata) is an important model organism for studying behavior, neuroscience, avian biology, and evolution. To support the study of its genome, we constructed a BAC library (TG__Ba) using DNA from livers of females. The BAC library consists of 147,456 clones with 98% containing inserts of an average size of 134 kb and represents 15.5 haploid genome equivalents. By sequencing a whole BAC, a full-length androgen receptor open reading frame was identified, the first in an avian species. Comparison of BAC end sequences and the whole BAC sequence with the chicken genome draft sequence showed a high degree of conserved synteny between the zebra finch and the chicken genome. |
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