|
|||||
|
|
| 杜仲叶多糖的提取分离、抗补体活性及结构研究 | |
| 引用本文： | 张学俊,伊廷金,孙黔云,张文坤,闫银萍,龚桂珍,宫本红. 杜仲叶多糖的提取分离、抗补体活性及结构研究[J]. 天然产物研究与开发, 2011, 23(4): 606-611,637 |
| 作者姓名： | 张学俊 伊廷金 孙黔云 张文坤 闫银萍 龚桂珍 宫本红 |
| 作者单位： | 1. 贵州省发酵工程与生物制药重点实验室贵州大学,贵阳,550003 2. 贵州省中国科学院天然产物化学重点实验室,贵阳,550002 3. 新时代健康产业有限公司,烟台,264006 |
| 摘 要： | 经石油醚脱脂、除胶后,采用热水提取、分步醇沉工艺获得杜仲叶水溶性多糖PsEUL1、PsEUL2、PsEUL3,分别经S-8大孔树脂脱色,Sevag法脱蛋白及透析,其中PsEUL1经DEAE-52纤维素柱层析分离得多糖组分PsEUL11、PsEUL12、PsEUL13,抗补体活性实验表明,其中有多个组分有不同程度的抗补体活性,并呈一定的剂量效应关系。活性多糖PsEUL13经硫酸-咔唑反应及气相色谱法测定,单糖组成为:L-鼠李糖、D-岩藻糖、D-阿拉伯糖、D-木糖、D-葡糖糖、D-半乳糖、糖醛酸,含量比为11.8∶1.6∶37.7∶4.2∶10.7∶12.8∶21.2。 |
| 关 键 词： | 杜仲叶 多糖 提取分离 抗补体活性 结构分析 |
Isolation, Anti-complement Activities, and Chemical Structures of Polysaccharide from Eucommia ulmoides Leaves |
|
| ZHANG Xue-jun,YI Ting-jin,SUN Qian-yun,ZHANG Wen-kun,YAN Yin-ping,GONG Gui-zhen,GONG Ben-hong. Isolation, Anti-complement Activities, and Chemical Structures of Polysaccharide from Eucommia ulmoides Leaves[J]. Natural Product Research and Development, 2011, 23(4): 606-611,637 | |
| Authors: | ZHANG Xue-jun YI Ting-jin SUN Qian-yun ZHANG Wen-kun YAN Yin-ping GONG Gui-zhen GONG Ben-hong |
| Affiliation: | ZHANG Xue-jun1,YI Ting-jin1,SUN Qian-yun2,ZHANG Wen-kun1,YAN Yin-ping2,GONG Gui-zhen1,GONG Ben-hong3 1Guizhou Province Key Laboratory of Fermentation Engineering & Biological Pharmacy,Guizhou University,Guiyang 550003,2The Key Laboratory of Chemistry for Natural Products,Guizhou Province and Chinese Academy of Sciences,Guiyang 550002,3The New Era Health Industry(group) Co.Ltd,Yantai 264006 |
| Abstract: | After removal of resin and gutta percha under petroleum ether reflux,Eucommia ulmoides Leaves were decocted with distilled water and the extract was concentrated and fractionally precipitated by adding the one-fold,two-fold and three-fold volumes of alcohol to obtain three groups of crude polysaccharides,PsEUL1,PsEUL2,and PsEUL3.Of them,the group of polysaccharides,PsEUL1,was decolourized on a macro-porous resin(S-8) column and deproteinized using Sevag reagent.After dialysis,the refined PsEUL3 was applied ... |
| Keywords: | Eucommia ulmoides Leaf polysaccharide isolation anti-complement activities molecular structures |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |