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Isolation and characterization of polymorphic microsatellites in European chestnut (Castanea sativa Mill.)
Authors:E J Buck  M Hadonou  C J James  D Blakesley  K Russell
Abstract:Thirteen polymorphic microsatellite loci were isolated from Castanea sativa (Mill.). Six contained dinucleotide repeats, six contained trinucleotide repeats, and one contained a compound microsatellite of a trinucleotide and a tetranucleotide repeat. The loci were characterized using C. sativa trees from three populations in the UK and the parents and six seedlings from a Turkish mapping population. The number of alleles revealed varied from two to 14 (mean = 5.15) per loci. Eight loci were found to be useful in the mapping family.
Keywords:Castanea sativa  European chestnut  microsatellite  SSR
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