cDNA Microarray Analysis of Changes in Gene Expression Associated with MPP+ Toxicity in SH-SY5Y Cells

cDNA microarray analysis of 1-methyl-4-phenyl-pyridinium (MPP⁺) toxicity (1 mM, 72 h) in undifferentiated SH-SY5Y cells identified 48 genes that displayed a signal intensity greater than the mean of all differentially expressed genes and a two-fold or greater difference in normalized expression. RT-PCR analysis of a subset of genes showed that c-Myc and RNA-binding protein 3 (RMB3) expression decreased by 50% after 72 h of exposure to MPP⁺ (1 mM) but did not change after 72 h of exposure to 6-hydroxydopamine (25 M), rotenone (50 nM), and hydrogen peroxide (600 M). Exposure of retinoic acid (RA)-differentiated SH-SY5Y cells to MPP⁺ (1 mM, 72 h) also resulted in a decrease in RMB3 expression and an increase in GADD153 expression. In contrast, c-Myc expression was slightly increased in RA-differentiated cells. Collectively, these data provide new insights into the molecular mechanisms of MPP⁺ toxicity and show that MPP⁺ can elicit distinct patterns of gene expression in undifferentiated and RA-differentiated SH-SY5Y cells.