A Fast Electrophoretic Isoenzyme Technique for the Identification of Invasive and Non-Invasive Entamoeba histolytica and E. hitolytica-like Organisms |
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| Authors: | DELYNN M. MOSS HENRY M. MATHEWS |
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| Affiliation: | U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control, Atlanta, Georgia 30333 |
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| Abstract: | Cellulose acetate electrophoresis (CAE) was used to separate glucosephosphate isomerase, hexokinase, malic enzyme, and phosphoglucomutase extracted from invasive and non-invasive Entamoeba histolytica and E. histolytica -like organisms. Each of these morphologically similar organisms possessed a unique CAE isoenzyme profile that can be used as an aid in their identification. The CAE technique used to obtain these isoenzyme profiles is rapid, simple, and economical, and it requires neither specialized training nor claborate equipment. |
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