The application of laser microprobe mass analysis to the study of biological material |
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Authors: | Theodore C Iancu Daniel P Perl Irmin Sternlieb Aaron Lerner Esther Leshinsky Edwin H Kolodny Amy Hsu Paul F Good |
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Institution: | (1) Pediatric Research Unit, Carmel Hospital and B. Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel;(2) The Arthur M. Fishberg Research Center for Neurobiology, Mount Sinai Medical Center, New York, NY, USA;(3) The National Center for the Study of Wilson's Disease, New York, NY, USA;(4) The Department of Neurology, New York University School of Medicine, New York, NY, USA;(5) Electron Microscopy Laboratories, B. Rappaport Faculty of Medicine, PO Box 9649, 31096 Haifa, Israel |
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Abstract: | Laser microprobe mass analysis (LAMMA) is an investigational method which is a powerful tool for the identification and quantitation of various elements present in small volumes of tissue. LAMMA is highly sensitive and capable of rapidly detecting concentrations of 1–3 p.p.m. of most metallic elements, in precisely localized cellular compartments. In order to further assess its value, cultured skin fibroblasts and biopsy tissues from human subjects and experimental animals were probed by LAMMA, and the results were correlated with ultrastructural findings. Biopsy samples were obtained from patients suffering from Gaucher disease, and from patients and animals with pathologic iron or copper metabolism. No significant abnormalities were detected in the cultured fibroblasts from patients with Gaucher disease, in contrast to the iron content of tissue biopsy Gaucher cells, which was markedly increased, apparently as a consequence of erythrophagocytosis. Particularly intense iron-related peaks were found in liver cytosiderosis due to neonatal or genetic haemochromatosis, thalassaemia major and in animal models of iron overload. An additional finding was the presence of aluminium accumulation in siderosomes of different cells. In liver biopsy samples from human Wilson's disease and from rats with an inherited disorder causing copper toxicosis, copper-containing compounds were identified and localized, and their relative concentration was estimated by LAMMA. The present study showed that LAMMA is a valuable technique for the localization and estimation of relative abundance of trace elements in various tissues containing excessive amounts of metals. |
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Keywords: | aluminium copper electron microscopy Gaucher disease iron laser microprobe mass spectrometry |
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