Role of a tachykinin-related peptide and its receptor in modulating the olfactory sensitivity in the oriental fruit fly,Bactrocera dorsalis (Hendel) |
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| Institution: | 1. Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China;2. Department of Crop Protection, Ghent University, Ghent, Belgium;1. Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, PR China;2. Academy of Agricultural Sciences, Southwest University, Chongqing 400715, PR China;3. Department of Crop Protection, Ghent University, 9000 Ghent, Belgium;1. Guangzhou City Key Laboratory of Subtropical Fruit Tree Outbreak Control, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, People’s Republic of China;2. Guangzhou Customs Technology Center, Guangzhou 510623, People’s Republic of China;1. Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China;2. Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000 Ghent, Belgium;1. Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China;2. Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China;3. Department of Crop Protection, Ghent University, Ghent, Belgium |
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| Abstract: | Insect tachykinin-related peptide (TRP), an ortholog of tachykinin in vertebrates, has been linked with regulation of diverse physiological processes, such as olfactory perception, locomotion, aggression, lipid metabolism and myotropic activity. In this study, we investigated the function of TRP (BdTRP) and its receptor (BdTRPR) in an important agricultural pest, the oriental fruit fly Bactrocera dorsalis. BdTRPR is a typical G-protein coupled-receptor (GPCR), and it could be activated by the putative BdTRP mature peptides with the effective concentrations (EC50) at the nanomolar range when expressed in Chinese hamster ovary cells. Consistent with its role as a neuromodulator, expression of BdTRP was detected in the central nervous system (CNS) of B. dorsalis, specifically in the local interneurons with cell bodies lateral to the antennal lobe. BdTRPR was found in the CNS, midgut and hindgut, but interestingly also in the antennae. To investigate the role of BdTRP and BdTRPR in olfaction behavior, adult flies were subjected to RNA interference, which led to a reduction in the antennal electrophysiological response and sensitivity to ethyl acetate in the Y-tube assay. Taken together, we demonstrate the impact of TRP/TRPR signaling on the modulation of the olfactory sensitivity in B. dorsalis. The result improve our understanding of olfactory processing in this agriculturally important pest insect. |
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| Keywords: | Oriental fruit fly Neuropeptide Tachykinin-related peptide G-protein coupled-receptor Olfaction |
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