Identification and quantitation of electron-transport components in human polymorphonuclear neutrophils |
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| Authors: | Carol C. Cunningham Lawrence R. DeChatelet Priscilla I. Spach J.Wallace Parce Michael J. Thomas Cynthia J. Lees Pamela S. Shirley |
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| Affiliation: | Department of Biochemistry, The Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, NC 27103 U.S.A. |
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| Abstract: | Using dithionite difference spectra we have detected cytochrome b in highly purified human neutrophils at a concentration of 0.08 nmol/mg protein. The presence of quinone was identified in lipid extracts at a concentration of approx. 0.06 nmol/mg protein. It was identified as ubiquinone-10 by mass spectrographic analysis. Simultaneous measurements of cytochrome oxidase indicated that these compounds could not be attributed to mitochondrial contamination. These results are compatible with the hypothesis that initiation of the respiratory burst in human neutrophils involves a multicomponent electron-transport system. |
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| Keywords: | Electron transport Ubiquinone Neutrophil Respiratory burst Cytochrome b (Human) |
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