Characterization of virus infectivity and cell-free capsid assembly of SIVMneCL8 |
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Authors: | Dooher Julia E Pineda Mario Javier Overbaugh Julie Lingappa Jaisri R |
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Institution: | Department of Pathobiology, University of Washington, Seattle, WA, USA. |
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Abstract: | We have previously described a cell-free system that reconstitutes immature capsid assembly of Gag polypeptides from viruses belonging to three major primate lentiviral lineages, including HIV-1, HIV-2 and SIVagm. Studies described here examine a member of the SIVmac/Mne lineage, SIVMneCL8, using assays for virus production and infectivity as well as cellular events in capsid formation. We report that SIVMneCL8, a molecular clone with properties typical of transmitted viral variants, is less infectious per unit p27 Gag than another member of the SIVmac/Mne lineage, SIVmac239. SIVMneCL8 Gag polypeptides are arrested at an early stage of capsid assembly in the cell-free system. Additionally, SIVMneCL8 Gag polypeptides associate minimally with the host factor human HP68. This is the first report of a primate lentivirus that does not complete capsid assembly in the cell-free system. |
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Keywords: | Gag Macaca nemestrina simian immunodeficiency virus SIVMne |
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