Tissue 3Vicia Faba

The establishment of a callus culture of Vicia faba root tissuegrowing on a modified Bonner and Devirian medium supplementedwith 10^-6 M 2,4-dichlorophenoxyacetic acid and 5.0 g/l yeastextract is reported. Experiments have been carried out to determinethe optimum growth conditions of the callus on semisolid mediaand an investigation made of the potential of this materialas an initial source of friable tissue yielding cell suspensionson dissociation in liquid medium. The development of two morphologicallydistinct types of callus tissue was observed on semi-solid mediumand chemical analysis has demonstrated a correlation betweenthe friability and cell-wall composition of these tissues.