Accumulation of multiacetylated forms of histones by trichostatin A and its developmental consequences in early starfish embryos |
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| Authors: | Susumu Ikegami Yasunori Ooe Takahiko Shimizu Toshihiko Kasahara Tatsuhiko Tsuruta Masako Kijima Minoru Yoshida Teruhiko Beppu |
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| Institution: | (1) Department of Applied Biochemistry, Hiroshima University, 4-4, Kagamiyama 1 Chome, 724 Higashi-Hiroshima, Hiroshima, Japan;(2) Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo, Yayoi 1-1-1, 113 Bunkyo-ku, Tokyo, Japan |
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| Abstract: | Summary External application of 10 rig/ml (R)-trichostatin A (TSA), a potent and specific inhibitor of mammalian histone deacetylase, to the embryo of the starfish Asterina pectinifera inhibited development during the early gastrula stage before formation of mesenchyme cells. The TSA-sensitive period was limited to the mid-blastula stage before hatching. The pulse-chase experiment clearly demonstrated that TSA induced an accumulation of acetylated histone species in blastulae through inhibition of historic deacetylation. Similar blockage of development at the early gastrula stage was observed with n-butyrate, which has been known as a weak inhibitor of historic deacetylase. These results suggest an intimate role for historic acetylation-deacetylation equilibria in starfish development.
Correspondence to: S. Ikegami |
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| Keywords: | Trichostatin A n-Butyric acid Histone acetylation Embryos Echinodermata |
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