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Immobilized pH gradient-driven paper-based IEF: a new method for fractionating complex peptide mixtures before MS analysis |
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| Authors: | Beerelli Seshi Kumaraguru Raja KH Chandramouli |
| Institution: | (1) Department of Pathology and Laboratory Medicine, Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, 1124 West Carson Street, Torrance, California 90502, USA;(2) , 580 Cross Point Parkway Getzville, NY 14221, USA;(3) Section of Marine Ecology and Biotechnology, Division of Life Science, The Hong Kong University of Science and Technology, Kowloon, Hong Kong SAR |
| Abstract: | IntroductionThe vast difference in the abundance of different proteins in biological samples limits the determination of the complete proteome of a cell type, requiring fractionation of proteins and peptides before MS analysis. |
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