Some cytochemical studies on the cell surface ofAmphidinium carterae (Dinophyceae) |
| |
Authors: | M Emilia Klut T Bisalputra N J Antia |
| |
Institution: | (1) Department of Zoology, University of British Columbia, Vancouver, B.C.;(2) Department of Botany, University of British Columbia, V6T 1W5 Vancouver, B.C., Canada;(3) West Vancouver Laboratory, Canadian Department of Fisheries and Oceans, West Vancouver, B.C. |
| |
Abstract: | Summary The surface coat of the dinoflagellateAmphidinium carterae Hulburt was examined by fluorescence and transmission electron microscopy, using various fluorochromes and cationic dyes. The overall results showed cell-surface reactions typical of acid mucopolysaccharides. The cationic dye staining revealed an outer fine fibrillar layer (15–70 nm thick) overlying a dense anionic coat (40–60 nm thick) which appeared to thicken progressively with age. In general, the structure of the amphiesmal vesicles was similar to that previously described by other investigators. However, an acidic mucopolysaccharide layer was observed on the inner surface of these vesicles. Each of these structures is traversed by 1–3 pores and at least 2 types of extrusomes are formed, the spindle trichocysts and the mucocysts. Cell to cell adhesion through the surface coat was frequently observed. Evidence was also obtained for internalization of all the surface-coat markers used.This investigation forms part of a doctoral thesis submitted by the first author to the University of British Columbia, Vancouver, B.C. |
| |
Keywords: | Amphidinium Amphiesma Cationic dyes Dinoflagellate Glycocalyx Lectins Mucopolysaccharide Trichocysts |
本文献已被 SpringerLink 等数据库收录! |
|