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| 水稻减数分裂染色体分析方法 | |
| 引用本文： | 程新杰,于恒秀,程祝宽. 水稻减数分裂染色体分析方法[J]. 植物学报, 2019, 54(4): 503-508. DOI: 10.11983/CBB19087 |
| 作者姓名： | 程新杰 于恒秀 程祝宽 |
| 作者单位： | 1 扬州大学农学院, 扬州 2250092 中国科学院遗传与发育生物学研究所植物基因研究中心, 北京 100101 |
| 摘 要： | 减数分裂粗线期染色体研究技术的发展, 很大程度上克服了水稻(Oryza sativa)细胞遗传研究中较小染色体所带来的研究困难。减数分裂染色体的制备与观察已经成为水稻细胞遗传学研究中的常规方法。该文详细描述了水稻中常用的减数分裂染色体制备、荧光原位杂交和免疫荧光染色的实验方法。 |
| 关 键 词： | 水稻 减数分裂 染色体 荧光原位杂交 免疫荧光染色 |
| 收稿时间： | 2019-05-12 |
Protocols for Analyzing Rice Meiotic Chromosomes |
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| Xinjie Cheng,Hengxiu Yu,Zhukuan Cheng. Protocols for Analyzing Rice Meiotic Chromosomes[J]. Bulletin of Botany, 2019, 54(4): 503-508. DOI: 10.11983/CBB19087 | |
| Authors: | Xinjie Cheng Hengxiu Yu Zhukuan Cheng |
| Affiliation: | 1 Agriculture College, Yangzhou University, Yangzhou 225009, China2 Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China |
| Abstract: | The development of techniques to analyze pachytene chromosomes has greatly overcome most of the difficulties in cytological studies of rice chromosomes caused by their small size. Visualization of meiotic chromosomes has now become routine in cytogenetic studies of this species. This chapter provides protocols on basic meiotic chromosome preparation, FISH analysis and immunocytology in rice. |
| Keywords: | rice meiosis chromosome FISH immunostaining |
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