Optimisation of the second phase of a two phase growth system for anthocyanin accumulation in callus cultures of Rudbeckia hirta |
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Authors: | Luczkiewcz M Cisowski W |
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Institution: | (1) Medical University of Gdańsk, Al. Gen. J, Hallera 107, 80-416 Gdansk-Wrzeszcz, Poland |
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Abstract: | A two-phase growth system composed of modified Schenk-Hildebrandt medium and enriched Miller's medium was used to grow callus
of R. hirta L. The second phase of the system has been optimised and the callus synthesised a 12-compound set of anthocyanins, comprising
4.97% of tissue mass (tubular flowers of the plant in nature only contain one compound comprising 0.28%). The beneficial effect
of high content of cysteine on anthocyanin accumulation was noted. The predominant element of the anthocyanin set obtained
in vitro is cyanidin-3-O-(6-O-malonyl-β-D-glucopyranoside), a relatively stable compound.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | Asteraceae cysteine malonated anthocyanins Rudbeckia hirta two-phase growth system |
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