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Biologically relevant effects of mRNA amplification on gene expression profiles |
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| Authors: | Rachel IM van Haaften Blanche Schroen Ben JA Janssen Arie van Erk Jacques JM Debets Hubert JM Smeets Jos FM Smits Arthur van den Wijngaard Yigal M Pinto Chris TA Evelo |
| Affiliation: | (1) BiGCaT Bioinformatics, University of Maastricht and Technical University Eindhoven, Maastricht, The Netherlands;(2) Experimental and Molecular Cardiology/ CARIM, University of Maastricht, Maastricht, The Netherlands;(3) Department of Pharmacology and Toxicology/ CARIM, University of Maastricht, Maastricht;(4) Department of Genetics and Cell Biology, University of Maastricht, Maastricht |
| Abstract: | BackgroundGene expression microarray technology permits the analysis of global gene expression profiles. The amount of sample needed limits the use of small excision biopsies and/or needle biopsies from human or animal tissues. Linear amplification techniques have been developed to increase the amount of sample derived cDNA. These amplified samples can be hybridised on microarrays. However, little information is available whether microarrays based on amplified and unamplified material yield comparable results. |
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