Alternative synthesis of corticotropin-like intermediate lobe peptide (human CLIP). A peptide corresponding to the sequence of human ACTH-(18--39)

A novel synthesis of human CLIP, a peptide corresponding to the sequence of human ACTH-(18-39) is described. The dodecapeptide chain was assembled by a combination of fragment condensation and stepwise synthesis while using N alpha-benzyloxycarbonyl and side chain tert.-butyl-derived protective groups combination. The final deprotection was performed by acidolysis in trifluoroacetic acid. The end product was purified by ion exchange chromatography on carboxymethyl cellulose.