Cosmid cloning and transposon mutagenesis in Salmonella typhimurium using phage lambda vehicles |
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| Authors: | E. Tapio Palva, Peter Liljeströ m Shigeaki Harayama |
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| Affiliation: | (1) Molecular Genetics Laboratory, Department of Genetics, University of Helsinki, SF-00100 Helsinki 10, Finland;(2) Membrane Group, Department of Molecular Biology, The Wallenberg Laboratory, University of Uppsala, S-75122 Uppsala, Sweden |
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| Abstract: | Summary We have constructed a strain of Salmonella typhimurium which contains the malB region from Escherichia coli and carries the bacteriophage  receptor protein in its outer membrane. Phage adsorbs to this strain but cannot grow, thus providing a very useful system for transposon mutagenesis of S. typhimurium using vehicles carrying transposons. This system can also be used for cosmid cloning. |
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