转抗菌肽D烟草对土壤微生物群落的影响

采用RAPD分子标记技术研究了种植转抗菌肽D烟草的土壤环境中微生物群落遗传多样性的变化,同时用传统平板培养法研究了土壤中可培养微生物在数量上的变化。RAPD分析结果表明,转抗菌肽D烟草与非转基因烟草根围微生物的遗传多样性相关指数并没有显著差异。培养计数结果表明,转抗菌肽D烟草与非转基因烟草根围可培养细菌在数量上有极显著差异,可培养真菌数量有显著减少,可培养放线菌的数量没有显著差异。说明转抗菌肽D烟草可能抑制了病原细菌及其根围相关的微生物,但是不影响微生物的遗传多样性。

Effects of transgenic tobacco with cecropin D on soil microbial communities

The study was carried out with both random amplified polymorphic DNA (RAPD) polymorphism with which to analyze the genetic diversity of soil microbial communities and cultured-based microbiological method with which to analyze the numerical change of soil microbial communities, in order to find the difference between the soil planted with transgenic tobacco with the cecropin D gene and the soil planted with non-transgenic tobacco. The results from RAPD showed that the difference of genetic diversity related index of microbial communities in two kinds of soils was not significant. But the result from culturing and numbering showed that transgenic tobacco with the cecropin D gene significantly reduced microbial colony number. The decrement is: bacterial> fungus. Actinomycetes population was not affected. The results suggested that transgenic tobacco with the cecropin D gene may inhibit the pathogenic bacterial and the related rhizospheric microbes, but does not affect the microbial genetic diversity.