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Lactoperoxidase-catalyzed iodinaton of cell cultures infected with mycoplasma
Authors:Karl W. Lanks  Nena W. Chin
Affiliation:(1) Department of Pathology, State University of New York, Downstate Medical Center, 450 Clarkson Avenue, 11203 Brooklyn, New York
Abstract:Summary Hamster BHK cells or secondary cultures of mouse embryo fibroblasts are not iodinated by lactoperoxidase in the absence of hydrogen peroxide. When such cell cultures are infected with a noncultivable strain ofM. hyorhinis, endogenous peroxide generation is sufficient to permit nearly maximal iodination. The SDS-polyacrylamide gel pattern of iodinated cell surface polypeptides is essentially the same regardless of the source of peroxide and whether or not the cultures are infected with mycoplasma.
Keywords:iodination  mycoplasma  hydrogen peroxide  cell surface proteins  SDS-polyacrylamide gel electrophoresis
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