Diosgenin,a plant steroid,induces apoptosis in human rheumatoid arthritis synoviocytes with cyclooxygenase-2 overexpression |
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Authors: | Email author" target="_blank">Bertrand?LiagreEmail author Pascale?Vergne-Salle Cecile?Corbiere Jean?L?Charissoux Jean?L?Beneytout |
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Institution: | Laboratoire de Biochimie, UPRES EA 1085, Faculté de Pharmacie, 2 rue du Docteur Marcland, 87025 Limoges Cedex, France. bertrand.liagre@unilim.fr |
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Abstract: | In the present study, we have shown for the first time that a plant steroid, diosgenin, causes an inhibition of the growth
of fibroblast-like synoviocytes from human rheumatoid arthritis, with apoptosis induction associated with cyclooxygenase-2
(COX-2) up-regulation. Celecoxib, a selective COX-2 inhibitor, provoked a large decrease in diosgenin-induced apoptosis even
in the presence of exogenous prostaglandin E2, whereas interleukin-1β, a COX-2 inducer, strongly increased diosgenin-induced apoptosis of these synoviocytes. These findings
suggest that the proapoptotic effect of diosgenin is associated with overexpression of COX-2 correlated with overproduction
of endogenous prostaglandin E2. We also observed a loss of mitochondrial membrane potential, caspase-3 activation, and DNA fragmentation after diosgenin
treatment. |
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