Detection and genetic characterization of rabies virus from human patients |
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Authors: | Wen-rong Yao Guo-qiang Pan Cheng-long Xiong Qian-fu Zhou Qi-you Xiao Ming-hui Li Yong-zhen Zhang |
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Institution: | 1. Department of Hemorrhagic Fever, Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China 2. The Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China 3. Xinning Center for Disease Control and Prevention, Xinning 422700, China 4. Hunan Center for Disease Control and Prevention, Changsha 410005, China |
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Abstract: | Saliva and blood were collected from two patients who had not received post exposure prophylaxis in the cities of Wenzhou
and Xinning respectively. Both patients were confirmed as positive for rabies by detection of rabies virus specific nucleoprotein
antibodies in the sera by Western Blot. However, rabies virus specific RNA was only identified in the saliva collected from
the patient in Wenzhou. Furthermore, the isolate Zhejiang Wz0 (H) was obtained by inoculating one-day-old suckling mice. Both
nucleoprotein (N) and glycoprotein (G) genes from the isolate were amplified by RT-PCR and sequenced. Phylogenetic analysis
indicated that the isolate belonged to classic rabies virus, and shared a higher homology with the street viruses from dogs
in the main endemic areas in China and the street virus from dogs in Indonesia than with other known strains. Further comparison
of the deduced amino acid sequences between the isolate and the vaccine strains used in China showed that the virus had a
higher level of homology with the vaccine strain CTN than with the other vaccine strains (3aG, PV, PM and ERA). In particular,
amino acid residues substitutions located in antigenic site III in the G protein, which could react with the neutralizing
antibodies, were observed. These results suggested that the virus belonged to the classic rabies virus, and both N and G genes
diverged from the current vaccine strains used in China at either the nucleotide or the amino acid level.
Foundation items: The project was supported by the Grants (2003BA712A08-02 and 2004BA718B03) from the Chinese Ministry of
Science and Technology. |
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Keywords: | Rabies Virus Nucleoprotein Gene Glycoprotein Gene Phylogenetic Analysis |
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