Screening for topoisomerase I binding compounds by high-performance liquid chromatography-mass spectrometry |
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| Authors: | Zhang Hong Gu Qing Liang Xinle Pan Yuanjiang |
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| Affiliation: | Department of Food Science, Hangzhou University of Commerce, Hangzhou, People's Republic of China. |
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| Abstract: | A new rapid compound screening approach for topoisomerase I binding activity is presented. DNA topoisomerase I is used as a target protein to capture binding compounds from a mixture of combinatorial compounds by bioaffinity ultrafiltration. Using high-performance liquid chromatography combined with electrospray ionization mass spectrometry, small-molecule active compounds were identified. We also have successfully applied this method to identifying compounds from cells grown in culture. |
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