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Interaction of hemin with quadruplex DNA |
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| Authors: | Maryam Ghahremani Nasab Leila Hassani Sara Mohammadi Nejad Davood Norouzi |
| Affiliation: | 1.Department of Biological Sciences,Institute for Advanced Studies in Basic Sciences (IASBS),Zanjan,Iran;2.Laboratory of Cell Biology,National Cancer Institute NIH,Bethesda,USA |
| Abstract: | A DNA enzyme with peroxidase activity is a G-quadruplex-based DNAzyme formed by hemin and G-quadruplex DNA. Activity of peroxide DNAzymes can be influenced by the structure of quadruplex DNA. In this investigation, the interaction of hemin with T30695 G-quadruplex DNA is evaluated. Molecular dynamic simulation indicates that the binding mode of hemin to G-quadruplex DNA is end-stacking, which is consistent with absorption spectroscopy. Based on fluorescence spectroscopy, hemin ejects thiazole orange from bases of four-strand DNA. Circular dichroism spectra showed that no alteration occurs in this type of DNA structure. Graphical Abstract Peroxidase DNAzyme is formed by hemin and G-quadruplex DNA. |
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