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Single cell transcriptomics of neighboring hyphae of |
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| Authors: | Charissa de Bekker Oskar Bruning Martijs J Jonker Timo M Breit Han AB Wösten |
| Affiliation: | (1) Microbiology and Kluyver Centre for Genomics of Industrial Fermentations, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands;(2) Microarray Department and Integrative Bioinformatics Unit, Swammerdam Institute for Life Sciences, University of Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands;(3) Netherlands Bioinformatics Centre (NBIC), Geert Grooteplein 28, 6525 GA Nijmegen Nijmegen, the Netherlands |
| Abstract: | Single cell profiling was performed to assess differences in RNA accumulation in neighboring hyphae of the fungus Aspergillus niger. A protocol was developed to isolate and amplify RNA from single hyphae or parts thereof. Microarray analysis resulted in a present call for 4 to 7% of the A. niger genes, of which 12% showed heterogeneous RNA levels. These genes belonged to a wide range of gene categories. |
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