Improving isothermal DNA amplification speed for the rapid detection of Mycobacterium tuberculosis期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Improving isothermal DNA amplification speed for the rapid detection of Mycobacterium tuberculosis

Authors:	Motré Aurélie Kong Richard Li Ying

Affiliation:	BioHelix Corporation, 500 Cummings Center, Suite 5550, Beverly, MA 01915, United States

Abstract:	In this study, we report the development of a helicase-dependent amplification assay for rapid detection of Mycobacterium tuberculosis (MTB). By applying a step-by-step optimization method, the amplification time from an input of 2-copy MTB genomic DNA was reduced from about 60 min to less than 30 min.

Keywords:	Helicase-dependent amplification (HDA) Isothermal Mycobacterium tuberculosis complex IS6110
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