CARD-FISH and confocal laser scanner microscopy to assess successional changes of the bacterial community in freshwater biofilms |
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| Authors: | Lupini Giuliano Proia Lorenzo Di Maio Mariarita Amalfitano Stefano Fazi Stefano |
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| Affiliation: | a Water Research Institute, IRSA-CNR, via Salaria km 29.300, 00015 Monterotondo, Roma, Italyb Institute of Aquatic Ecology, University of Girona, Campus Montilivi, 17071, Girona, Spain |
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| Abstract: | Bacterial community composition was assessed during riverine biofilm development by the Catalyzed Reporter Deposition Fluorescence In Situ Hybridization (CARD-FISH) in combination with Confocal Laser Scanning Microscopy. Using artificial substrates, it was possible to follow the dynamics of specific bacterial clusters, while maintaining the unaltered structure and architecture of the biofilm. |
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| Keywords: | Biofilm Bacterial community composition CARD-FISH CLSM Stream ecology |
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