Development of a modified DNA extraction method for pulsed-field gel electrophoresis analysis of Staphylococcus aureus and enterococci without using lysostaphin期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Development of a modified DNA extraction method for pulsed-field gel electrophoresis analysis of Staphylococcus aureus and enterococci without using lysostaphin

Authors:	Feizabadi Mohammad Mehdi Ghodousi Arash Nomanpour Bizhan Omrani Maryam Shahcheraghi Fereshteh

Institution:	^a Department for Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran ^b Department of Bacteriology, Pasteur Institute of Iran, 12th of Farvardin Street, Tehran, Iran

Abstract:	A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Staphylococcus aureus and enterococci to reduce the cost of using lysostaphin. This protocol reduces the expenses of PFGE typing of S. aureus and enterococci as it removes the use of lysostaphin during the spheroplast formation from these bacteria.

Keywords:	Pulse-field gel electrophoresis(PFGE) MRSA VRE Nosocomial infections
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