An atomic force microscopy method for the detection of binding forces between bacteria and a lipid bilayer containing higher order gangliosides期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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An atomic force microscopy method for the detection of binding forces between bacteria and a lipid bilayer containing higher order gangliosides

Authors:	Adams Elizabeth L Almagro-Moreno Salvador Boyd E Fidelma

Institution:	^a Delaware Biotechnology Institute, University of Delaware, Newark, DE, USA ^b Department of Biological Sciences, University of Delaware, Newark, DE, USA

Abstract:	We developed an atomic force microscopy (AFM) method to determine the binding forces between a model cell wall plasma membrane and Vibrio cholerae. V. cholerae cells were covalently attached to AFM probes and forces were determined against a lipid bilayer containing sialic acid (N-acetylneuraminic acid) molecules as well as several control surfaces.

Keywords:	AFM atomic force microscope APTES 3-aminopropylmethyldiethoxysilane SAM self assembled monolayer PBS phosphate buffered saline HBS HEPES buffered saline POPC lipids palmitoyl-oleyl-sn-phosphatidyl-choline
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