Distinct differentiation of closely related species of Bacillus subtilis group with industrial importance |
| |
| Authors: | Jeyaram Kumaraswamy Romi Wahengbam Singh Thangjam Anand Adewumi Gbenga Adedeji Basanti Khundrakpam Oguntoyinbo Folarin Anthony |
| |
| Institution: | a Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD), Takyelpat Institutional Area, Imphal-795001, Manipur, Indiab Department of Microbiology, Faculty of Science, University of Lagos, Akoka, Lagos, Nigeria |
| |
| Abstract: | PCR amplification of 16S rRNA gene by universal primers followed by restriction fragment length polymorphism analysis using RsaI, CfoI and HinfI endonucleases, distinctly differentiated closely related Bacillus amyloliquefaciens, Bacillus licheniformis and Bacillus pumilus from Bacillus subtilis sensu stricto. This simple, economical, rapid and reliable protocol could be an alternative to misleading phenotype-based grouping of these closely related species. |
| |
| Keywords: | Bacillus subtilis group In silico analysis ARDRA API 50CHB ITS-RFLP |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
|
