High-throughput DNA isolation method for detection of Xylella fastidiosa in plant and insect samples |
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| Authors: | Brady Jeff A Faske Jennifer B Castañeda-Gill Jessica M King Jonathan L Mitchell Forrest L |
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| Affiliation: | a Texas AgriLife Research, Stephenville, TX, United Statesb University of Texas, San Antonio, San Antonio, TX, United Statesc University of North Texas Health Science Center, Fort Worth, TX, United States |
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| Abstract: | ![]()
We report an inexpensive, high-throughput method for isolating DNA from insect and plant samples for the purpose of detecting Xylella fastidiosa infection. Existing methods often copurify inhibitors of DNA polymerases, limiting their usefulness for PCR-based detection assays. When compared to commercially available kits, the method provides enhanced pathogen detection at a fraction of the cost. |
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| Keywords: | Pierce's disease Glassy-winged sharpshooter qPCR Yellow sticky trap |
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