环境水体中肠道感染菌群与几种典型致病菌的QPCR检测及相关性分析

利用定量PCR(QPCR)方法,对环境水体中的肠道感染菌群及几种致病菌进行4个月的连续检测。结果表明:在污染严重的水体中,埃希氏大肠菌(E.coli)是水中肠道感染菌群的主体,其强度接近于用通用引物检测出的肠道感染菌群强度。当肠道感染菌群拷贝数超过10⁴copies·100 mL^-1时,沙门氏菌(S.typhrmurium)和志贺氏痢疾杆菌(Shigella flexneri)也持续被检出,可以认为是水体病原污染的标志之一;在轻度污染和清洁水体中,当肠道感染菌群拷贝数低于10⁴copies·100 mL^-1时,通过通用引物检测到的肠道感染菌群主要是上述3种病原细菌以外的细菌,且3种病原细菌的出现频度和强度与肠道感染菌群之间没有明显的相关性。

Detection and correlativity analysis of enteric infectious flora and three typical pathogenic bacteria in surface waters by QPCR

The copies of enteric infectious flora and three typical pathogenic bacteria in surface waters were detected continuously for four months by using quantitative PCR(QPCR) method.The results show that in the seriously polluted waters,E.coli is dominant among the enteric infectious flora,and the copies of E.coli are almost the same as enteric infectious flora detected by universal primers.When the copies of enteric infectious flora in water are more than 10⁴ copies·100mL^-1,S.typhrmurium and Shigella flexneri can be detected continuously,which can be considered as one of the indicators of water pathogenic pollution.In the clean and slightly polluted waters,when the copies of enteric infectious flora are less than 10⁴ copies·100mL^-1,the enteric pathogens are mainly other bacteria except the three kinds of typical pathogenic bacteria mentioned above.The copies and detection frequency of the three kinds bacteria have no significant correlation with the enteric infectious flora monitored by universal primers.