大型真菌冬菇103工业发酵产物及生物活性的研究

本研究表明大型真菌冬菇103 Flammulina velutipes(Curt.: Fr.)Sing.-103工业发酵性能良好,具备生产周期短、产量高、产物种类多等特点,发酵时间72—96小时,在深层发酵培养基中,每升可形成9—15g干菌丝体;菌丝体的稠密度为每升含有40—42g干物质。并能产生多种功能的生理活性物质。从菌丝体和发酵液中分析出16种氨基酸、14种微量元素、4种维生素、其中包括脂溶性维生素E醋酸酯、αE、K_1和水溶性维生素B_2此外还提取出具有抗菌活性和免疫功能的粗多糖和菌丝干粉(包括菌丝的提取液),它们在抗菌活性方面对大肠杆菌(Eschericha coli);八叠球菌(Sarcina later);枯草杆菌(Bacillus subtilis);油桐炭疽菌(Colletotrichum Sp.c-837-1)显示出不同程度的抗菌活性。在促进免疫功能方面,可促进小鼠吞噬鸡红血球功能和促进体液免疫小鼠溶血空斑的形成,菌丝干粉经小鼠急性毒性LD_(50)测定表明,小鼠以5g/kg剂量为最高档剂,给药后未见死亡,故103菌丝干粉对小鼠的最大安全量为5g/kg以上。

STUDIES ON PRODUCTS OF INDUSTIAL FERMENTATION AND THEIR BIOLOGICAL ACTIVITY OF FLAMMULINA VELUTIPES 103

The study indicates that Flammulina velutipes 103 is with the capacity of industrial fermentation. It has the characteristics of short production cycle, high yield and a great variety of products. The fermentation time is 72-96 hrs. the deep fermentation culture could produce 9-15g/L dried mycelium; The density of the mycelium is 40-42g/L dried substances. And they can produce some substances having a wide variety of function and physiological activity. From the mycelium and the fermentation liquid have analized 16 different amino acids, 14 trace elements, 4 kinds of vitamins, including fat soluble vitarmin aE and water soluble vitarmin B2. In addition to these, The authors also extracted the crude polysaccharide and liquid which has antibacterial activity and immune function. They show various degree of antibacterial activity to coccobacillus (Eschericha coll), sarcinae (Sarcina lutea), subtiline (Bacillus subtilis) and tung tree anthracnose disease (Colletotrichum sp. C-837-1). In promotion of immune fuction, they can promote the function of the mouse engulfing cock erythrocyte to form and promote the mouse having humoral immunity to form the hemolytic plaque in its body. The acute toxicity of dried hypha powder to the mouse, tested by LD50, indicates that after giving the medicine at the dose of 5g/kg as the maximum the death of mouse has not been seen. Therefore the maximum safe dose of dried hypha powder 103 to mouse is above 5g/kg.