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Chemical evaluation of compounds as nitric oxide or peroxynitrite donors using the reactions with serotonin |
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| Authors: | Blanchard B Servy C Ducrocq C |
| Affiliation: | a Department of Experimental Medicine, University of Genova, Italyb Department of Internal Medicine, University of Genova, Italy |
| Abstract: | It has been suggested that diabetes induces an increase in oxidative stress; the increased expression of heme-oxygenase 1 (HO-1) in liver is believed to be a sensitive marker of the stress response. The aim of this study was to examine whether diabetes is able to induce HO-1 expression in liver. The specific mRNA was amplified by RT/PCR and calibrated with amplified β-actin mRNA. The mRNA HO-1 levels in the liver of spontaneously diabetic rats were increased by 1.8 fold compared with non diabetics; this supports the hypothesis of weak but significant oxidative damage due to chronic hyperglycaemia. This work represents the first in vivo study exploring the semi-quantitative expression of HO-1 in the liver of spontaneously diabetic rats. |
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