Isolation of a new paramutagenic allele of thesulfurea locus in the tomato cultivar Moneymaker following in vitro culture |
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Authors: | E Wisman M S Ramanna M Koornneef |
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Institution: | (1) Department of Plant Breeding, Agricultural University, P.O. Box 386, NL-6700 AJ Wageningen, The Netherlands;(2) Department of Molecular Biology, Agricultural University, Dreyenlaan 3, NL-6703 HA Wageningen, The Netherlands;(3) Department of Genetics, Agricultural University, Dreyenlaan 2, NL-6703 HA, Wageningen, The Netherlands |
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Abstract: | A new allele, SC148, of thesulfurea locus inLycopersicon esculentum was detected in a line derived after repeated selfing of plants that had been regenerated from tissue culture. Like the originalsulf mutant, SC148 displayed two mutant phenotypes: green-yellow speckled plants in which thesulf
vag allele is present and pure yellow plants homozygous for thesulf
tpura allele. Although the mutant alleles are recessive to wild-type, an unpredictable number of variegated and pura plants appeared in F1 progenies that had been derived from crosses between SC148 and wild-type tomato plants. The presence of the wild-typesulf
+ allele in these variegated heterozygotes was demonstrated using a cytological marker that is linked tosulf. It is concluded that the mutantsulf allele of SC148, imposes its variegated expression state on the wild-typesulf
+ allele present insulf
+/sulfvag heterozygotes. This behaviour, known as paramutation, has also been described for the originalsulf allele. The SC148 allele, however, seems to induce changes at an earlier stage in development. The analogy of this paramutagenic system to dominant position effect variegation inDrosophila is discussed. |
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Keywords: | Sulfurea Tomato Paramutation Dominant position effect variegation |
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