A lectin on the hemocyte membrane of the oyster (Crassostrea virginica) |
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| Authors: | Gerardo R. Vasta Thomas C. Cheng John J. Marchalonis |
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| Affiliation: | 1. Department of Biochemistry, Medical University of South Carolina, 171 Ashley Avenue, Charleston, South Carolina 29425 U.S.A.;2. Marine Biomedical Research Program and Department of Anatomy (Cell Biology), Medical University of South Carolina, 171 Ashley Avenue, Charleston, South Carolina 29425 U.S.A. |
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| Abstract: | Using antisera produced against a serum lectin we have shown by employing immunocytofluorescence that hemocytes from the oyster, Crassostrea virginica, possess a lectin which is situated on the external surface of the cell membrane. The antisera block the binding of hemocyte microsomes to protease-treated vertebrate erythrocytes, thus confirming that the hemocyte membrane lectin is serologically related to the serum lectin. The major serum lectin has an apparent mass of 34,000. Flow cytometry has revealed that the distribution of the surface lectin on hemocytes represents a heterogeneous expression on a population basis, but no discrete cell subpopulations can be identified. |
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| Keywords: | To whom correspondence should be sent at the address given above. G.R.V. is associated with the Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina and recipient of the International Fogarty Fellowship Award IF 05TW031155 (National Institutes of Health). |
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