| 杜仲剥皮再生过程中可溶性蛋白和过氧化物酶含量及分布的变化(英文) |
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| 引用本文： | HOUHong-Wei Kalima-N＇KomaMWANGE WANGYa-Qing CUIKe-Ming. 杜仲剥皮再生过程中可溶性蛋白和过氧化物酶含量及分布的变化(英文)[J]. Acta Botanica Sinica, 2004, 46(2): 216-223 |
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| 作者姓名： | HOUHong-Wei Kalima-N＇KomaMWANGE WANGYa-Qing CUIKe-Ming |
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| 作者单位： | 北京大学生命科学学院，北京大学生命科学学院，北京大学生命科学学院，北京大学生命科学学院，北京大学生命科学学院 北京 100871，北京 100871 High Commission of Atomic Energy/Regional Center ofNuclear Research，B.P，868.Kinshasa 11，D.R.Congo，北京 100871 High Commission of Atomic Energy/Regional Center ofNuclear Research，B.P，868.Kinshasa 11，D.R.Congo，北京 100871，北京 100871 |
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| 基金项目： | 国家重点基础研究发展规划项目(G1999016002)，国家自然科学基金(30170056，30070614)~~ |
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| 摘 要： | Peroxidases are known to play important roles in plant wound healing. Biochemical analysisand histochemical localization techniques were used to assess changes and distribution of peroxidases inthe recovering bark after girdling in Eucommia ulmoides Oliv. Between 4 and 21 days after girdling (DAG),peroxidases activity in the girdled trees significantly increased by 30-40 times over that in ungirdled trees.During the whole bark recovery process (from 0 to 63 DAG), the peroxidase signal was not found in thetissue regions subjected to intense cell division activity (regenerating cambial zone and phellogen). However,high peroxidase activity was detected in the callus, cortex-like, mature phloem and xylem. Interestingly, itwas shown that, in maturing xylem and phloem cells, there was respectively an inward and outwardperoxidase activity gradient on both sides of the cambium zone. An isoelectric-focusing electrophoresis ofthe extracted protein displayed two isozyme bands of peroxidase: POD Ⅰ and POD Ⅱ. POD Ⅰ was onlydetected in the xylem fraction and could play a role in xylem differentiation. POD Ⅱ was only identified inthe recovering bark portion and could be more engaged in bark regeneration process. A relationshipbetween IAA and peroxidase is also discussed.
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| 关 键 词： | 过氧化物酶 形成层 杜仲 剥皮 再生 同工酶 |
Changes of Soluble Protein, Peroxidase Activity and Distribution During Regeneration After Girdling in Eucommia ulmoides |
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| HOU Hong-Wei,Kalima-N'Koma MWANGE,WANG Ya-Qing,CUI Ke-Ming. Changes of Soluble Protein, Peroxidase Activity and Distribution During Regeneration After Girdling in Eucommia ulmoides[J]. , 2004, 46(2): 216-223 |
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| Authors: | HOU Hong-Wei Kalima-N'Koma MWANGE WANG Ya-Qing CUI Ke-Ming |
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| Affiliation: | CollegeofLifeSciences,PekingUniversity,Beijing100871,China |
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| Abstract: | |
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| Keywords: | peroxidase cambium Eucommia ulmoides girdling regeneration isozyme |
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