| 用实时荧光PCR方法鉴定转基因玉米T14/T25 |
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| 引用本文： | 曹际娟,覃文,朱水芳,曹远银.用实时荧光PCR方法鉴定转基因玉米T14/T25[J].遗传,2004,26(5):689-694. |
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| 作者姓名： | 曹际娟 覃文 朱水芳 曹远银 |
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| 作者单位： | 1.沈阳农业大学，沈阳 110161；2.辽宁出入境检验检疫局，大连 116001；3.广州出入境检验检疫局，广州 510623；4.国家质检总局动植物检疫实验所，北京 100029 1. Shenyang Agriculture University , Shenyang 110161, China; 2. Liaoning Entry-Exit Inspection and Quarantine Bureau, Dalian 116001, China; 3. Guangzhou Entry-Exit Inspection and Quarantine Bureau, Guangzhou 510623, China; 4. Institute of Animal and Plant Quarantine, AQSIQ, Beijing 100029, China |
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| 基金项目： | 国家转基因植物研究和产业化专项基金 |
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| 摘 要： | 本研究以实时荧光PCR技术鉴定商业化种植的转基因玉米T14/T25品系。根据转基因玉米T14/T25转入的外源基因质粒图谱，设计转基因引物和探针进行PCR和实时荧光PCR检测，建立了转基因玉米品系鉴定的实时荧光PCR方法。实验结果表明，用TaqMan探针可检测到T14/T25产生的荧光信号，而对其他玉米品系则检测不到荧光信号，为转基因产品的鉴定检测提供了新方法。Abstract: To identify genetically modified (GM) maize T14/T25 lines, a real-time fluorescent PCR (RTF PCR) assay was performed in this study. Primers and Taqman probes specific for inserted genes in the T14/T25 were used to conduct the real-time fluorenscent (RTF) PCR and PCR assays. The RTF PCR method was established to detect and identify GM maize lines. The results show that the TaqMan probe could identify T14/T25 maize used, while other GM and NO-GM maize didn’t be detected. The RTF PCR could be a new method for detecting other genetically modified organism.
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| 关 键 词： | real-time fluorescent PCR 鉴定Key words T14/T25 GM maize T14/T25转基因玉米 实时荧光PCR |
| 文章编号： | 0253-9772(2004)05-0689-06 |
| 修稿时间： | 2003年7月7日 |
Identification for Genetically Modified Maize T14/T25 with Real Time Fluorenscent PCR Method |
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| CAO Ji-Juan.Identification for Genetically Modified Maize T14/T25 with Real Time Fluorenscent PCR Method[J].Hereditas,2004,26(5):689-694. |
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| Authors: | CAO Ji-Juan |
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| Institution: | Shenyang Agriculture University, Shenyang 110 161, China. cjj0909@sina.com |
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| Abstract: | To identify genetically modified (GM) maize T14/T25 lines,a real-time fluorescent PCR (RTF PCR) assay was performed in this study. Primers and Taq man probes specific for inserted genes in the T14/T25 were used to conduct the real-time fluorenscent (RTF) PCR and PCR assays.The RTF PCR method was established to detect and identify GM maize lines.The results show that the Taq Man probe could identify T14/T25 maize used,while other GM and NO-GM maize didn't be detected.The RTF PCR could be a new method for detecting other genetically modified organism. |
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| Keywords: | real-time fluorescent PCR T14/T25 GM maize identification |
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