Effect of thyrotropin on glycosaminoglycans synthesized by primocultured thyroid cells |
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Authors: | Annie Giraud Simone Bouchilloux |
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Institution: | Laboratoire de Biochimie Médicale et U 38 INSERM, Faculté de Médecine, 27 Bd J. Moulin, F-13385 Marseille Cédex 5, France |
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Abstract: | The synthesis of glycosaminoglycans (GAGs) was investigated in porcine thyroid cells under the influence or not of thyrotropin. After labelling with 3H] glucosamine and 35S] , enriched GAG-fractions prepared from culture media, cells, and eventually substrate adhering materials, were analyzed by cellulose acetate electrophoresis combined with specific degradations. They comprised heparan sulfate and hyaluronic acid together with an unknown sulfated component labile to endo-β-galactosidase. Whereas global labellings of newly made GAGs were not significantly modified by thyrotropin, we reproducibly observed with the hormone a substantial increase in the proportion of hyaluronic acid 3H] label and, when cells organized into follicles, of the proportion of cell-associated 3H] GAGs. This system thus offers an interesting model to study how the responsiveness to an hormone and the reorganization that follows might implicate specific glycoconjugates. |
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Keywords: | TSH thyrotropin GAG glycosaminoglycan HA hyaluronic acid HS heparan sulfate MEM Eagle's minimum essential medium PBM phosphate-buffered medium 137 mM NaCl 2 7 mM KCl 5 mM benzamidine SAM substrate attached material |
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