New Insight into the Thermal Properties and the Biological Behaviour of the Bacterial Spores |
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Authors: | Hue Nguyen Thi Minh Jean-Marie Perrier-Cornet Patrick Gervais Dominique Champion |
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Institution: | 1.Laboratoire de Génie des Procédés Microbiologiques et Alimentaires Agrosup Dijon,Université de Bourgogne,Dijon,France;2.Equipe “Eau—Molécules actives—Macromolécules—Activités” Agrosup Dijon,Université de Bourgogne,Dijon,France |
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Abstract: | The physicochemical properties of spores were studied in relationship of their structure, which was modulated by chemical
or genetic methods. The Bacillus subtilis spores were equilibrated at different water activities (from 0.113 to ~1) and investigated by differential scanning calorimetry
(DSC). The isothermal sorptions at 25 °C of the native and the modified spores were also used to analyse the DSC results.
As already reported in literature, an endothermic peak in DSC was found at about 70 °C, but a previously unreported baseline
shift, a ∆Cp step, was also observed at −69 °C. The endothermic peak found at 70 °C was assigned to a material relaxation
which corresponded to a structure change from a less mobile state to a more mobile state. The spore cortex material seems
to be mainly implicated in this event. The ∆Cp step observed at −69 °C was identified as a glass transition of the water in
the spore protoplast. These results showed that at room temperature, the physical state of the components within B. subtilis spores equilibrated at water activity levels below 0.3 was different: The cortex material is in a low mobility state whereas
confined structure of protoplast and its internal hydration level allow a certain mobility of water molecules. |
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